pGEX-4T-1 dATG1 (1188)
(Plasmid
#66918)
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Purposebacterial expression of dATG1
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-4T-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATG1
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SpeciesD. melanogaster (fly)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1 dATG1 (1188) was a gift from Michael Karin & Jun Hee Lee (Addgene plasmid # 66918 ; http://n2t.net/addgene:66918 ; RRID:Addgene_66918) -
For your References section:
Drosophila Fip200 is an essential regulator of autophagy that attenuates both growth and aging. Kim M, Park HL, Park HW, Ro SH, Nam SG, Reed JM, Guan JL, Lee JH. Autophagy. 2013 Aug;9(8):1201-13. doi: 10.4161/auto.24811. Epub 2013 May 14. 10.4161/auto.24811 PubMed 23819996
