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G protein alpha-s-HaloTag
(Plasmid #66993)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66993 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNAI/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 7295
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G-alpha-s-EE-HaloTag
  • Alt name
    Gnas
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2485
  • Mutation
    BamHI sites in the alpha-s cDNA were removed and alpha-s residues 73-84 were replaced with Gly-Ser adding a unique BamHI site into which HaloTag was subcloned.
  • Entrez Gene
    Gnas (a.k.a. ALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HaloTag flanked by SGGGGSGGGGS on each side was inserted in place of alpha-s residues 73- 84.
    • internal EE epitope (residues 189-194 in alpha- s sequence are EYMPTE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Alpha-s-EE was produced in the laboratory of Henry Bourne, University of California, San Francisco, CA. HaloTag pHT2 was purchased from Promega.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G protein alpha-s-HaloTag was a gift from Catherine Berlot (Addgene plasmid # 66993)