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pET-24a-ELP[V-150]
(Plasmid #67015)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67015 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-24a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5310
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ELP[V-150]
  • Species
    Synthetic
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (not destroyed)
  • 3′ cloning site BsaI (not destroyed)
  • 5′ sequencing primer AGATCTCGATCCCGCGAAATTAATACGACT
  • 3′ sequencing primer CCAACTCAGCTTCCTTTCGGGCTTT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-24a-ELP[V-150] was a gift from Ashutosh Chilkoti (Addgene plasmid # 67015 ; http://n2t.net/addgene:67015 ; RRID:Addgene_67015)
  • For your References section:

    Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins. Tang NC, Chilkoti A. Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521. 10.1038/nmat4521 PubMed 26726995