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Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3
(Plasmid #67276)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67276 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Amp
  • Backbone size w/o insert (bp) 2000
  • Total vector size (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP (mWasabi)
  • Insert Size (bp)
    700
  • Promoter CMV
  • Tag / Fusion Protein
    • F3 (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe (unknown if destroyed)
  • 3′ cloning site XcmI (unknown if destroyed)
  • 5′ sequencing primer TCAACCTGGAGGTGAAGGAG
  • 3′ sequencing primer TCAAGACCATCTACAGGGCC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    hygro
  • Insert Size (bp)
    1000
  • Tag / Fusion Protein
    • Frt (N terminal on backbone)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site MfeI (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)
  • 5′ sequencing primer GCCCAAAGCATCAGCTCATC
  • 3′ sequencing primer ATTTCGGCTCCAACAATGTC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3 was a gift from Jannik Elverløv-Jakobsen (Addgene plasmid # 67276 ; http://n2t.net/addgene:67276 ; RRID:Addgene_67276)