Purposemiddle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67696||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
Mutationum200, no stop codon; N1056S
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
The N1056S mutation in flt4 does not affect protein function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pME-Fv2E-flt4um200-ns was a gift from Nathan Lawson (Addgene plasmid # 67696)
For your References section:Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors. Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND. Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12. 10.1242/dev.137901 PubMed 27621059