pTmaM4
(Plasmid #67714)

• Purpose: Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain protein
• Depositing Labs: Robert Batey, Jeffrey Kieft
• Publication: Kieft et al RNA. 2004 Jun;10(6):988-95.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5708
• Total vector size (bp): 6000
• Modifications to backbone: Insertion between NcoI and BamHI sites
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Thermotoga marítima Ffh-M domain
• Species: Thermotoga marítima
• Insert Size (bp): 500
• Promoter: T7 RNA polymerase
• Tag / Fusion Protein:
  • hexahistidine tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry