pABD823
(Plasmid
#67911)
-
PurposeProtein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepBD-GAL4 Cam
-
Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 6494
- Total vector size (bp) 7646
-
Vector typeYeast Expression
-
Selectable markersTRP1
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCold temperature germinating 10 (CTG10)
-
SpeciesA. thaliana (mustard weed)
-
Insert Size (bp)1152
-
GenBank IDDQ666277
- Promoter pADH1 (alcohol dehydrogenase 1 promoter)
-
Tag
/ Fusion Protein
- GAL4 DNA binding domain (148 amino acids) (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer 5'BD
- 3′ sequencing primer 3'BD (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pABD823 was a gift from Bruce Downie (Addgene plasmid # 67911 ; http://n2t.net/addgene:67911 ; RRID:Addgene_67911) -
For your References section:
KELCH F-BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME-INTERACTING FACTOR1. Majee M, Kumar S, Kathare PK, Wu S, Gingerich D, Nayak NR, Salaita L, Dinkins R, Martin K, Goodin M, Dirk LMA, Lloyd TD, Zhu L, Chappell J, Hunt AG, Vierstra R, Huq E, Downie AB. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4120-E4129. doi: 10.1073/pnas.1711919115. Epub 2018 Apr 9. 10.1073/pnas.1711919115 PubMed 29632208