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0.6 hA33 luciferase
(Plasmid #68150)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68150 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    A33
  • Alt name
    GPA33
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    560
  • Mutation
    starts at -595 relative to ATG
  • GenBank ID
    10223
  • Entrez Gene
    GPA33 (a.k.a. A33)
  • Promoter human GPA33 promoter
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer lucNRev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    0.6 hA33 luciferase was a gift from Joan Heath (Addgene plasmid # 68150 ; http://n2t.net/addgene:68150 ; RRID:Addgene_68150)
  • For your References section:

    Analysis of the regulation of the A33 antigen gene reveals intestine-specific mechanisms of gene expression. Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, Viars CS, Czekay S, Arden KC, Heath JK. J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11. 10.1074/jbc.M204865200 PubMed 12114523