hRAD18 dSAP-EGFP
(Plasmid
#68825)
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PurposeExpresses human RAD18 (deleting SAP domain) tagged with EGFP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68825 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N3
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman RAD18 (deleting SAP domain) tagged with EGFP
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SpeciesH. sapiens (human)
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Entrez GeneRAD18 (a.k.a. RNF73)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer 5' primer for pEGFP-N3
- 3′ sequencing primer 3' primer for pEGFP-N3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hRAD18 dSAP-EGFP was a gift from Satoshi Tateishi (Addgene plasmid # 68825 ; http://n2t.net/addgene:68825 ; RRID:Addgene_68825) -
For your References section:
Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Tsuji Y, Watanabe K, Araki K, Shinohara M, Yamagata Y, Tsurimoto T, Hanaoka F, Yamamura K, Yamaizumi M, Tateishi S. Genes Cells. 2008 Apr;13(4):343-54. doi: 10.1111/j.1365-2443.2008.01176.x. 10.1111/j.1365-2443.2008.01176.x PubMed 18363965