pEGFP N1 Ub-CRT
(Plasmid #69619)

• Purpose: Express cytoplasmic mature CRT.
• Depositing Lab: Marta Hallak
• Publication: Goitea et al J Biol Chem. 2015 Jun 26;290(26):16403-14. doi: 10.1074/jbc.M114.626127. Epub 2015 May 12.

Backbone

• Vector backbone: pEGFP N1
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6160
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitina-Calreticulin (mature)
• Alt name: Ub-CRT
• Species: H. sapiens (human)
• Insert Size (bp): 1428
• Mutation: Deletion of signal peptide of calreticulin at the N-terminus (17aa)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Ubiquitin (N terminal on insert)
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: CGGGGTACCCCCAGCTCGTCCTTGGCCTGGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry