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Addgene

pUV15-pHGFP
(Plasmid #70045)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70045 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    unknown
  • Vector type
    Mycobacterium tuberculosis expression vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    phGFP
  • Alt name
    pH-sensitive ratiometric GFP
  • Species
    Synthetic
  • Insert Size (bp)
    700
  • Promoter mycobacterial promoter Psmyc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer psmyc1 CGACCAGCACGGCATACATC
  • 3′ sequencing primer pBluescript-KS
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pH-GFP insert in this plasmid contains a F220L mutation compared to the wild-type pHluorin. This difference does not affect function of the pH-GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUV15-pHGFP was a gift from Sabine Ehrt (Addgene plasmid # 70045 ; http://n2t.net/addgene:70045 ; RRID:Addgene_70045)
  • For your References section:

    A membrane protein preserves intrabacterial pH in intraphagosomal Mycobacterium tuberculosis. Vandal OH, Pierini LM, Schnappinger D, Nathan CF, Ehrt S. Nat Med. 2008 Aug;14(8):849-54. doi: 10.1038/nm.1795. Epub 2008 Jul 20. 10.1038/nm.1795 PubMed 18641659