pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)
(Plasmid #70232)

• Purpose: ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope
• Depositing Lab: Steven Henikoff
• Publication: Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.

Backbone

• Vector backbone: pFA6a-3HA-HIS3MX6
• Modifications to backbone: The 3HA coding sequencing was dropped out of backbone with AscI and PacI and replaced with 3FLAG-MNase.
• Vector type: Yeast genomic targeting
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 3xFLAG tag and aa 83-231 of Mnase
• Alt name: ChEC-tagging vector
• Alt name: 3FLAG-MNase
• Insert Size (bp): 525

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's QC results indicate the selection marker may be a different version of HIS than found in the full sequence, but the depositor notes this does not affect function.