Purpose(Empty Backbone) ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||70282||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonesee comments
- Backbone size (bp) 11127
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pRL542 comprises ColK-K35, GenBank AY929248.1 (replicon); portions of transposon Tn1 from IncPalpha Birmingham, GenBank BN000925.1; pSa (fragment bearing chloramphenicol-resistance determinant), GenBank L06822.4; and polylinker from pIC20H, GenBank L08912.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRL542 was a gift from Peter Wolk (Addgene plasmid # 70282 ; http://n2t.net/addgene:70282 ; RRID:Addgene_70282)
For your References section:Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP. J Bacteriol. 1997 Mar;179(6):1998-2005. PubMed 9068647