pBS/U6/RNAi gamma-tubulin shRNA mouse
(Plasmid #70666)

• Purpose: Reduced the expression of gamma-tubulin in murine cells
• Depositing Lab: Maria Alvarado-Kristensson
• Publication: Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25.

Backbone

• Vector backbone: pBluescript/U6
• Vector type: Mammalian Expression ; U6 promoter

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´
• Alt name: CDCBM4, GCP-1, TUBG, TUBGCP1; AI504772, Tubgl
• gRNA/shRNA sequence: TUBG1 and TUBG2
• Species: M. musculus (mouse)
• GenBank ID: NM_134024.2 NM_134028.2
• Entrez Gene: Tubg1 (a.k.a. 1500010O08Rik, GCP-1, Tubg)
• Entrez Gene: Tubg2 (a.k.a. AI504772, Tubg, Tubgl)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ApaI (destroyed during cloning)
• 3′ cloning site: ApaI (destroyed during cloning)
• 5′ sequencing primer: 5’-TTACATGATAGGCTTGGAT-3’

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there is a single nucleotide mismatch between the depositor's sequence and Addgene's quality control sequence. This mismatch does not affect plasmid function.