|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71181||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeInsect Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
- Promoter DSCP Drosophila synthetic core promoter
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSGE_886_Nhe2_EcR-to-ato_RB was a gift from Alexander Stark (Addgene plasmid # 71181 ; http://n2t.net/addgene:71181 ; RRID:Addgene_71181)
For your References section:Transcriptional regulators form diverse groups with context-dependent regulatory functions. Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A. Nature. 2015 Nov 9. doi: 10.1038/nature15545. 10.1038/nature15545 PubMed 26550828