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pUAST-GFP-RpL10Ab
(Plasmid #71729)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71729 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RpL10Ab
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    RpL10Ab (a.k.a. Dmel_CG7283, BcDNA:RH06366, CG7283, Dmel\CG7283, L10Ab, RpL10A, RpL10Ae, anon-EST:Posey188)
  • Promoter hsp70
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 5 ' restriction site used for cloning is NotI (EcoRI, BglII are 5' to that site) and there is an internal XhoI site at bp 84 of the 654 bp RpL10Ab fragment. There is a unique KpnI site immediately following the XhoI site at the 3' end of the cloned fragment. The NotI site and BglII sites are also usable for anyone wanting to reclone the fragment and the XhoI site is not usable but the KpnI site is.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-GFP-RpL10Ab was a gift from Herman Dierick (Addgene plasmid # 71729 ; http://n2t.net/addgene:71729 ; RRID:Addgene_71729)
  • For your References section:

    A versatile method for cell-specific profiling of translated mRNAs in Drosophila. Thomas A, Lee PJ, Dalton JE, Nomie KJ, Stoica L, Costa-Mattioli M, Chang P, Nuzhdin S, Arbeitman MN, Dierick HA. PLoS One. 2012;7(7):e40276. doi: 10.1371/journal.pone.0040276. Epub 2012 Jul 6. 10.1371/journal.pone.0040276 PubMed 22792260