pUAST-GFP-RpL10Ab
(Plasmid #71729)

• Purpose: Expression of Drosophila melanogaster Rpl10Ab-GFP fusion
• Depositing Lab: Herman Dierick
• Publication: Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371/journal.pone.0040276. Epub 2012 Jul 6.

Backbone

• Vector backbone: pUAST
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RpL10Ab
• Species: D. melanogaster (fly)
• Entrez Gene: RpL10Ab (a.k.a. Dmel_CG7283, BcDNA:RH06366, CG7283, Dmel\CG7283, L10Ab, RpL10A, RpL10Ae, anon-EST:Posey188)
• Promoter: hsp70
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: pCasper-hs
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 5 ' restriction site used for cloning is NotI (EcoRI, BglII are 5' to that site) and there is an internal XhoI site at bp 84 of the 654 bp RpL10Ab fragment. There is a unique KpnI site immediately following the XhoI site at the 3' end of the cloned fragment. The NotI site and BglII sites are also usable for anyone wanting to reclone the fragment and the XhoI site is not usable but the KpnI site is.