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UCP3 promoter EP1
(Plasmid #71743)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71743 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL4.12[luc2CP]
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4421
  • Total vector size (bp) 11467
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Uncoupling Protein 3 promoter
  • Alt name
    UCP3 promoter
  • Alt name
    Slc25a9 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7042
  • Mutation
    Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2
  • Entrez Gene
    Ucp3 (a.k.a. AI645527, Slc25a9, UCP-3)
  • Promoter UCP3
  • Tag / Fusion Protein
    • Luciferase

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer RV3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UCP3 promoter EP1 was a gift from Heinrich Taegtmeyer (Addgene plasmid # 71743 ; http://n2t.net/addgene:71743 ; RRID:Addgene_71743)
  • For your References section:

    Chronic Hyperinsulinemia Causes Selective Insulin Resistance and Downregulates Uncoupling Protein 3 Through the Activation of SREBP-1 Transcription Factor in Mouse Heart. Harmancey R, Haight DL, Watts KA, Taegtmeyer H. J Biol Chem. 2015 Nov 10. pii: jbc.M115.673988. 10.1074/jbc.M115.673988 PubMed 26555260