Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #71809)


Item Catalog # Description Quantity Price (USD)
Plasmid 71809 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    (GenBank EF362409.1)
  • Backbone size w/o insert (bp) 8052
  • Total vector size (bp) 10645
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Insert Size (bp)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Brian Sauer - pBS302 (Addgene plasmid# 11925)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] plasmid vector was constructed from the following elements: Drosophila minimal hsp70 promoter (“min.hsp70P”), STOP#1 cassette flanked by two unidirectional FRT sites, open reading frame encoding Dam-Myc fusion protein, polylinker consisting of AscI, SwaI, FseI, AsiSI and NotI sites, SV40 polyadenylation signal, attB site for phiC31 integrase-mediated transgenesis, pUC backbone, Drosophila mini-white marker and loxP site. The elements were assembled in the indicated order; the last five elements were taken as a whole from pUASTattB vector (Bischof et al., 2007, PMID=17360644; GenBank EF362409.1). The p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] vector is designed for cloning and expression of FLP-inducible N-terminal Dam fusion proteins.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] was a gift from Bas van Steensel (Addgene plasmid # 71809 ; ; RRID:Addgene_71809)
  • For your References section:

    Inducible DamID systems for genomic mapping of chromatin proteins in Drosophila. Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van Steensel B. Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 10.1093/nar/gkw176. Epub 2016 Mar 21. 10.1093/nar/gkw176 PubMed 27001518