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pTSTEP
(Plasmid #72334)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72334 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTL2
  • Backbone manufacturer
    PMID: 25555988
  • Backbone size w/o insert (bp) 7580
  • Total vector size (bp) 9740
  • Modifications to backbone
    gRNA cloning site changed to BsmBI from SapI. I-CreI and attPx sites removed.
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RRS-TagRFPT-p10-[Golic+] RRS-EGFP
  • Alt name
    T-STEP cassette
  • Insert Size (bp)
    1436

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pTL2 backbone from Chen et al, 2015, PMID: 25555988

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTSTEP was a gift from Avital Rodal (Addgene plasmid # 72334 ; http://n2t.net/addgene:72334 ; RRID:Addgene_72334)
  • For your References section:

    Tissue-specific tagging of endogenous loci in Drosophila melanogaster. Koles K, Yeh AR, Rodal AA. Biol Open. 2015 Dec 23. pii: bio.016089. doi: 10.1242/bio.016089. 10.1242/bio.016089 PubMed 26700726