D282N Cus2p
(Plasmid #73060)

• Purpose: S. cerevisiae Cus2p gene with D282N Mutation
• Depositing Lab: Aaron Hoskins
• Publication: Rodgers et al RNA. 2015 Dec 2.

Backbone

• Vector backbone: p11
• Backbone manufacturer: Midwest Center for Structural Genomics
• Backbone size w/o insert (bp): 5704
• Total vector size (bp): 6538
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CUS2p
• Alt name: U2 snRNP complex subunit CUS2
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 858
• Mutation: Asp 282 to Asn
• GenBank ID: 855430
• Entrez Gene: CUS2 (a.k.a. YNL286W)
• Promoter: T7
• Tag / Fusion Protein:
  • 6x His tag fused to TEV cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: TGGATGCTGTAGGCATAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry