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pETM6-G6-vioABE-4A6-vioC-3A2-vioD
(Plasmid #73440)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73440 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETM6
  • Backbone manufacturer
    Koffas Lab, Addgene Plasmid #49795
  • Backbone size w/o insert (bp) 5147
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PG6-vioABE-P4A6-vioC-P3A2-vioD
  • Species
    Pseudoalteromonas luteoviolacea strain B
  • Promoter PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

Cloning Information

Resource Information

Depositor Comments

Coexpression with G6 CRISPR repressor shuts off all colored metabolite production. Coexpression with 4A6 CRISPR repressor switches carbon flux from violacein production (violet culture) to proviolacein (blue-violet culture). Coexpression with with 3A2 CRISPR repressor switches carbon flux from violacein production (violet culture) to deoxyviolacein (red-violet).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM6-G6-vioABE-4A6-vioC-3A2-vioD was a gift from Mattheos Koffas (Addgene plasmid # 73440 ; http://n2t.net/addgene:73440 ; RRID:Addgene_73440)
  • For your References section:

    Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, Linhardt RJ, Koffas MA. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. 10.1093/nar/gkw231 PubMed 27079979