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C321.deltaA (Isaacs lab)
(Bacterial strain #73581)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 73581 Bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    This is a strain
  • Growth instructions
    Growth above 34 C is not recommended, since growth at elevated temperatures leads to leaky expression of toxic proteins from the lambda prophage.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    none
  • Alt name
    C321.ΔA.1
  • Alt name
    C321.deltaA.1

Resource Information

  • Terms and Licenses

Depositor Comments

E. coli MG1655 ΔmutS:zeo.Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla], ΔprfA; all 321 UAG codons changed to UAA

This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin. This is the exact strain used for protein expression in publication PMID:26571098.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321.deltaA (Isaacs lab) was a gift from Farren Isaacs (Addgene plasmid # 73581)
  • For your References section:

    Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW, Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll D, Rinehart J, Isaacs FJ. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16. 10.1038/nbt.3372 PubMed 26571098