FH95pUP95S73DGW (F3)
(Plasmid
#74040)
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Purposelentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusion
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneFUGW
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Backbone manufacturerDavid Baltimore (Addgene plasmid # 14883)
- Backbone size w/o insert (bp) 9941
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Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePsd95
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gRNA/shRNA sequenceGGA CAT CCA GGC ACA CAA G
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SpeciesR. norvegicus (rat)
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MutationS73D
- Promoter H1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BstBI (destroyed during cloning)
- 5′ sequencing primer H1 tcgctatgtgttctgggaaa (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene did not confirm the presence of the S73D mutation.
Map: FHUGW-(pUb)-HinDIII-SalI-XbaI-(p95S73D)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FH95pUP95S73DGW (F3) was a gift from Robert Malenka & Bernardo Sabatini & Weifeng Xu (Addgene plasmid # 74040 ; http://n2t.net/addgene:74040 ; RRID:Addgene_74040) -
For your References section:
Destabilization of the postsynaptic density by PSD-95 serine 73 phosphorylation inhibits spine growth and synaptic plasticity. Steiner P, Higley MJ, Xu W, Czervionke BL, Malenka RC, Sabatini BL. Neuron. 2008 Dec 10;60(5):788-802. doi: 10.1016/j.neuron.2008.10.014. 10.1016/j.neuron.2008.10.014 PubMed 19081375