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mRFP-Lact-C2
(Plasmid #74061)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74061 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mRFP-C1
  • Backbone size w/o insert (bp) 4646
  • Total vector size (bp) 5121
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lactadherin-C2
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    475
  • Entrez Gene
    MFGE8
  • Promoter CMV
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Sergio Grinstein obtained gene material, which was incorporated into the plasmid, from Dr. Gary Gilbert at Harvard University without any obligations or restrictions attached.
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-Lact-C2 was a gift from Sergio Grinstein (Addgene plasmid # 74061 ; http://n2t.net/addgene:74061 ; RRID:Addgene_74061)
  • For your References section:

    Membrane phosphatidylserine regulates surface charge and protein localization. Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A, Grinstein S. Science. 2008 Jan 11. 319(5860):210-3. 10.1126/science.1152066 PubMed 18187657