mRFP-Lact-C2
(Plasmid #74061)

• Purpose: Fluorescent biosensor for phosphatidylserine.
• Depositing Lab: Sergio Grinstein
• Publication: Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Backbone

• Vector backbone: mRFP-C1
• Backbone size w/o insert (bp): 4646
• Total vector size (bp): 5121
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lactadherin-C2
• Species: B. taurus (bovine)
• Insert Size (bp): 475
• Entrez Gene: MFGE8
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Sergio Grinstein obtained gene material, which was incorporated into the plasmid, from Dr. Gary Gilbert at Harvard University without any obligations or restrictions attached.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry