pCDNA3-HA-human MYCN
(Plasmid #74163)

• Purpose: Mammalian expression of human MycN
• Depositing Lab: Martine Roussel
• Publication: Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi: 10.1016/j.ccell.2015.12.003.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	74163	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pCDNA3
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5440
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MycN
• Species: H. sapiens (human)
• Entrez Gene: MYCN (a.k.a. MODED, N-myc, NMYC, ODED, bHLHe37)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Terms and Licenses:
  • UBMTA
• Article Citing this Plasmid:
  • 1 Reference