PurposeMammalian expression of tandem fusion of mClover3-mRuby3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74252||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerMichael Davidson Lab
- Total vector size (bp) 6778
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1473
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BshTI (unknown if destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer pshuttle5
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, T38V, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.
The specific substitutions used in the development of mClover3 relative to its parent Clover are N149Y, G160C, and A206K.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKanCMV-mClover3-mRuby3 was a gift from Michael Lin (Addgene plasmid # 74252 ; http://n2t.net/addgene:74252 ; RRID:Addgene_74252)
For your References section:Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J. Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. 10.1038/srep20889 PubMed 26879144