Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #74300)


Item Catalog # Description Quantity Price (USD)
Plasmid 74300 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4728
  • Total vector size (bp) 6030
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    treacle ribosome biogenesis factor 1
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Tcof1 (a.k.a. AA408847, AW209012, treacle)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site PstI (unknown if destroyed)
  • 5′ sequencing primer GFP-F
  • 3′ sequencing primer pEGFP-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene sequencing found extra LVPNSCSPGDPLVLERPAVPSTVPRARDPPDLDN on the C-terminus of the Tcof1 translation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NRCoC was a gift from Rita Shiang (Addgene plasmid # 74300 ; ; RRID:Addgene_74300)
  • For your References section:

    The Treacher Collins syndrome (TCOF1) gene product, treacle, is targeted to the nucleolus by signals in its C-terminus. Winokur ST, Shiang R. Hum Mol Genet. 1998 Nov;7(12):1947-52. ddb229 [pii] PubMed 9811939