MI-Luciferase
(Plasmid
#75018)
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PurposeEncodes the expression of Luciferase (with out any fluorescent reporter)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneMIGR1
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7150
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Modifications to backboneGFP is excised from MIGR1 vector
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Vector typeRetroviral, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefirefly Luciferase
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Alt nameLuc2
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Insert Size (bp)1653
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer TAC ATC GTG ACC TGG GAA GCC TTG G (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MI-Luciferase was a gift from Xiaoping Sun (Addgene plasmid # 75018 ; http://n2t.net/addgene:75018 ; RRID:Addgene_75018) -
For your References section:
Tyrosine kinase inhibitors induce mesenchymal stem cell-mediated resistance in BCR-ABL+ acute lymphoblastic leukemia. Mallampati S, Leng X, Ma H, Zeng J, Li J, Wang H, Lin K, Lu Y, Yang Y, Sun B, Gong Y, Lee JS, Konopleva M, Andreeff M, Arlinghaus RB, Cai Z, Fang B, Shen H, Han X, Hirsch-Ginsberg CF, Gao X, Paranjape AN, Mani SA, Clise-Dwyer K, Sun X. Blood. 2015 May 7;125(19):2968-73. doi: 10.1182/blood-2014-05-576421. Epub 2015 Feb 23. 10.1182/blood-2014-05-576421 PubMed 25712988