pJK057
(Plasmid #75036)

• Purpose: Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
• Depositing Labs: T. J. Kappock, JoAnne Stubbe
• Publication: Purine biosynthesis (unpublished)

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 5191
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: N5-carboxyaminoimidazole ribonucleotide mutase
• Alt name: purE
• Alt name: JW0512
• Alt name: b0523
• Species: Escherichia coli str. K-12 substr. MG1655
• Mutation: changes residues 2-7 to His (SSRNNP>HHHHHH)
• GenBank ID: NP_415056
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: N5-carboxyaminoimidazole ribonucleotide synthetase
• Alt name: purK
• Alt name: JW0511
• Alt name: b0522
• Species: Escherichia coli
• GenBank ID: NP_415055
• Entrez Gene: purK (a.k.a. b0522, ECK0515, JW0511, purE2)
• Promoter: n/a

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by T. J. Kappock in the laboratory of JoAnne Stubbe (MIT).

In XL1Blue cells.