PurposePlasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||75439||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backbonereplaced pLtetO promoter with arabinose inducible pBAD promoter
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
- Promoter pBAD
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CTCGAGAAGAAACCAATTGTC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZA16mflon was a gift from James Collins (Addgene plasmid # 75439 ; http://n2t.net/addgene:75439 ; RRID:Addgene_75439)
For your References section:Tunable protein degradation in bacteria. Cameron DE, Collins JJ. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17. 10.1038/nbt.3053 PubMed 25402616