PurposeCe cha-1 expression in motor neurons in worms
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78194||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesC. elegans (nematode)
- Promoter unc-3
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer GFP R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJL1604 was a gift from Junho Lee (Addgene plasmid # 78194 ; http://n2t.net/addgene:78194 ; RRID:Addgene_78194)
For your References section:Nictation, a dispersal behavior of the nematode Caenorhabditis elegans, is regulated by IL2 neurons. Lee H, Choi MK, Lee D, Kim HS, Hwang H, Kim H, Park S, Paik YK, Lee J. Nat Neurosci. 2011 Nov 13;15(1):107-12. doi: 10.1038/nn.2975. 10.1038/nn.2975 PubMed 22081161