Purposeoptogenetic activation of IL2 neuron in worms
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78195||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3684
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode)
- Promoter lag-2
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer pGEX 3 (Common Sequencing Primers)
Terms and Licenses
This plasmid contains 1-51 of Uncharacterized protein CELE_F32A7.6 (NP_493594.1). The depositor states that this should not adversely affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJL1702 was a gift from Junho Lee (Addgene plasmid # 78195 ; http://n2t.net/addgene:78195 ; RRID:Addgene_78195)
For your References section:Nictation, a dispersal behavior of the nematode Caenorhabditis elegans, is regulated by IL2 neurons. Lee H, Choi MK, Lee D, Kim HS, Hwang H, Kim H, Park S, Paik YK, Lee J. Nat Neurosci. 2011 Nov 13;15(1):107-12. doi: 10.1038/nn.2975. 10.1038/nn.2975 PubMed 22081161