MetNI (Lm)
(Plasmid #78316)

• Purpose: Methionine ABC Transporter
• Depositing Lab: Douglas Rees
• Publication: Kadaba et al Science. 2008 Jul 11;321(5886):250-3. doi: 10.1126/science.1157987.

Backbone

• Vector backbone: pET19b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5791
• Total vector size (bp): 7471
• Modifications to backbone: Added extra 10x Histidine Tag to accomodate second insert (MetN)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: MetI
• Species: E. coli
• Insert Size (bp): 651
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • 6x Histidine Tag (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Gene/Insert 2

• Gene/Insert name: MetN
• Species: E. coli
• Insert Size (bp): 1029
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • 10x Histidine Tag (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry