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pDGE5
(Plasmid #79461)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79461 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Modifications to backbone
    BsaI site (in bla) eliminated
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pAtU6-ccdB-sgRNA
  • gRNA/shRNA sequence
    none, empty sgRNA shuttle vector
  • Insert Size (bp)
    1498
  • Mutation
    BsaI site eliminated
  • Entrez Gene
    ccdB(letD) (a.k.a. D616_p97105, Fpla045)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGE5 was a gift from Johannes Stuttmann (Addgene plasmid # 79461 ; http://n2t.net/addgene:79461 ; RRID:Addgene_79461)
  • For your References section:

    Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. 10.1111/tpj.13319 PubMed 27579989