pNR64
(Plasmid #79540)

• Purpose: Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.
• Depositing Lab: Timothy Lu
• Publication: Roquet et al Science. 2016 Jul 22;353(6297):aad8559. doi: 10.1126/science.aad8559. Epub 2016 Jul 21.

Backbone

• Vector backbone: Dual-Recombinase-Controller
• Backbone manufacturer: Addgene Plasmid #44456
• Total vector size (bp): 6591
• Modifications to backbone: Replaced camR with KanR.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alphaPRO
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: TP901
• Tags / Fusion Proteins:
  • His tag (N terminal on insert)
  • LAA degradation tag (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: BxbI

Resource Information

• Supplemental Documents:
  • GenBank
• A portion of this plasmid was derived from a plasmid made by: The plasmid is the Dual-Recombinase-Controller (addgene plasmid 44456) from the Endy Lab, modified to have KanR instead of CamR on the backbone.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The DH5alphaPRO strain is DH5alpha modified to express LacI and TetR constitutively.