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CIBN-GFP-TMD
(Plasmid #79571)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79571 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    syntaxin 1A
  • Species
    M. musculus (mouse)
  • Mutation
    aa 249_288 (C-terminal region (polybasic stretch and transmembrane domain from human Syntaxin 1A)
  • Entrez Gene
    Stx1a (a.k.a. HPC-1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • CIBN (N terminal on backbone)
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (not destroyed)
  • 3′ cloning site BsrG1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains the C-terminal region (polybasic stretch and transmembrane domain; amino acids 249-288) from human Syntaxin 1A (NM_004603.3).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CIBN-GFP-TMD was a gift from Pietro De Camilli & Olof Idevall-Hagren (Addgene plasmid # 79571 ; http://n2t.net/addgene:79571 ; RRID:Addgene_79571)
  • For your References section:

    Optogenetic control of phosphoinositide metabolism. Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30. 10.1073/pnas.1211305109 PubMed 22847441