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pT2/SVNeo-103
(Plasmid #79841)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79841 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT2/SVNeo Addgene #26553
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 9500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RpBphP1-mCherry-Intersectin1 DHPH domain
  • Species
    Rhodopseudomonas palustris
  • Insert Size (bp)
    3700
  • GenBank ID
    NCBI Gene rpa1537 KX063613
  • Promoter CMVd1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer ggaaacagctatgaccatg
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    A RpBphP1 gene of R. palustris (NCBI Gene rpa1537) was provided by E. Giraud (Institute for Research and Development, France).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

New paper providing detailed protocol for using this plasmid- https://www.nature.com/articles/nprot.2018.022

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2/SVNeo-103 was a gift from Vladislav Verkhusha (Addgene plasmid # 79841 ; http://n2t.net/addgene:79841 ; RRID:Addgene_79841)
  • For your References section:

    A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Kaberniuk AA, Shemetov AA, Verkhusha VV. Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864. 10.1038/nmeth.3864 PubMed 27159085