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pU6-SAG1-DHFR Citations (4)

Originally described in: A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.
Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC, Lourido S Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
PubMed Journal

Articles Citing pU6-SAG1-DHFR

Articles
CRISPR-Cas9-based genome-wide screening of Toxoplasma gondii. Sidik SM, Huet D, Lourido S. Nat Protoc. 2018 Jan;13(1):307-323. doi: 10.1038/nprot.2017.131. Epub 2018 Jan 11. PubMed
Optimizing Systems for Cas9 Expression in Toxoplasma gondii. Markus BM, Bell GW, Lorenzi HA, Lourido S. mSphere. 2019 Jun 26;4(3). pii: 4/3/e00386-19. doi: 10.1128/mSphere.00386-19. PubMed

Associated Plasmids

Identification of a Master Regulator of Differentiation in Toxoplasma. Waldman BS, Schwarz D, Wadsworth MH 2nd, Saeij JP, Shalek AK, Lourido S. Cell. 2020 Jan 23;180(2):359-372.e16. doi: 10.1016/j.cell.2019.12.013. Epub 2020 Jan 16. PubMed
Genome-wide CRISPR screen identifies genes synthetically lethal with GRA17, a nutrient channel encoding gene in Toxoplasma. Paredes-Santos TC, Bitew MA, Swale C, Rodriguez F, Krishnamurthy S, Wang Y, Maru P, Sangare LO, Saeij JPJ. PLoS Pathog. 2023 Jul 27;19(7):e1011543. doi: 10.1371/journal.ppat.1011543. eCollection 2023 Jul. PubMed

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