|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80678||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneBinary vector pAMPAT-MCS
Modifications to backboneintroduction of firefly Luciferase 3' of Gateway site to create C-terminal fusions
Vector typePlant Expression
- Promoter Pro35S
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsccdB Survival can also be used for tranformation
Copy numberLow Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer gaccggcaacaggattcaatcttaag
- 3′ sequencing primer GAATTAGAAATTTTATTGATAGAAG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM-PAT-35S-GW-Luc was a gift from Nico Dissmeyer & Imre Somssich (Addgene plasmid # 80678 ; http://n2t.net/addgene:80678 ; RRID:Addgene_80678)