Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMA-SpCas9-g6
(Plasmid #80789)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80789 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMA15
  • Backbone manufacturer
    Lifetechnologies
  • Backbone size w/o insert (bp) 2380
  • Total vector size (bp) 2789
  • Modifications to backbone
    no
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPR gRNA expression cassette (for SpCas9)
  • gRNA/shRNA sequence
    empty (BbsI)
  • Species
    Synthetic
  • Promoter U6
  • Tag / Fusion Protein
    • na

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA-SpCas9-g6 was a gift from Yonglun Luo (Addgene plasmid # 80789 ; http://n2t.net/addgene:80789 ; RRID:Addgene_80789)
  • For your References section:

    Golden Gate Assembly of CRISPR gRNA expression array for simultaneously targeting multiple genes. Vad-Nielsen J, Lin L, Bolund L, Nielsen AL, Luo Y. Cell Mol Life Sci. 2016 May 13. 10.1007/s00018-016-2271-5 [pii] PubMed 27178736