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Pmyo-3::tag:SL1(G20A)
(Plasmid #81114)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 81114 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEASY-Blunt Simple Cloning Vector
  • Backbone manufacturer
    Transgen
  • Backbone size w/o insert (bp) 3830
  • Total vector size (bp) 6364
  • Vector type
    Worm Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sls-1.2
  • Alt name
    SL1
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2354
  • Mutation
    G20A
  • Entrez Gene
    sls-1.2 (a.k.a. CELE_Y102A5D.14)
  • Promoter promoter of myo-3
  • Tag / Fusion Protein
    • illumina-adapter (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Joel H. Rothman's lab (Mol Cell Biol. 1999 Mar;19(3):1892-900)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pmyo-3::tag:SL1(G20A) was a gift from Xiao Liu (Addgene plasmid # 81114 ; http://n2t.net/addgene:81114 ; RRID:Addgene_81114)
  • For your References section:

    Analysis of C. elegans muscle transcriptome using trans-splicing-based RNA tagging (SRT). Ma X, Zhan G, Sleumer MC, Chen S, Liu W, Zhang MQ, Liu X. Nucleic Acids Res. 2016 Aug 23. pii: gkw734. 10.1093/nar/gkw734 PubMed 27557708