This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p.UTA.2.0 Empty
(Plasmid #82446)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82446 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    psiCHECK™-2 Vector
  • Backbone manufacturer
    Promega
  • Backbone size (bp) 3660
  • Modifications to backbone
    Luciferase genes were replaced with CFP and YFP. YFP after the Tk Promoter and CFP after te SV40 promoter. Other regions were changes to full fill correct ORF for the fluorescent proteins and full fill cloning requirements.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Depositor Comments

Please see the following published protocol for details on plasmid use and data analysis: https://bio-protocol.org/e3000

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p.UTA.2.0 Empty was a gift from Jens Gruber (Addgene plasmid # 82446 ; http://n2t.net/addgene:82446 ; RRID:Addgene_82446)
  • For your References section:

    Dissecting miRNA gene repression on single cell level with an advanced fluorescent reporter system. Lemus-Diaz N, Böker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M & Gruber J. Scientific Reports 7, Article number: 45197 (2017) doi:10.1038/srep45197