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p.UTA.2.0 Empty
(Plasmid #82446)


Item Catalog # Description Quantity Price (USD)
Plasmid 82446 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
    psiCHECK™-2 Vector
  • Backbone manufacturer
  • Backbone size (bp) 3660
  • Modifications to backbone
    Luciferase genes were replaced with CFP and YFP. YFP after the Tk Promoter and CFP after te SV40 promoter. Other regions were changes to full fill correct ORF for the fluorescent proteins and full fill cloning requirements.

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Depositor Comments

Please see the following published protocol for details on plasmid use and data analysis:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p.UTA.2.0 Empty was a gift from Jens Gruber (Addgene plasmid # 82446 ; ; RRID:Addgene_82446)
  • For your References section:

    Dissecting miRNA gene repression on single cell level with an advanced fluorescent reporter system. Lemus-Diaz N, Böker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M & Gruber J. Scientific Reports 7, Article number: 45197 (2017) doi:10.1038/srep45197