Addgene - pFUGW-H1 empty vector Plasmid Data

Plasmid 25870: pFUGW-H1 empty vector

Gene/insert name:
None
Vector backbone:
FUGW-H1
(Search Vector Database)
Backbone manufacturer:
Ivanova, NB
Vector type:
Lentiviral
Backbone size (bp):
10130
Cloning site 5':
Xba1
Site destroyed during cloning:
No
Cloning site 3':
Sma1
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Sally Temple
Terms and Licenses:
MTA

Comments:

Empty Vector pFUGW-H1

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development. Fasano et al (Cell Stem Cell. 2007 Jun 7. 1(1):87-99. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25870" in your Materials and Methods section.