Addgene - Bmi1-overexpression Plasmid Data

Plasmid 21577: Bmi1-overexpression

Gene/insert name:
Bmi-1
Insert size:
2500
Species:
M. musculus (mouse)
GenBank ID:
NM_007552
Entrez Gene:
Bmi1 (RP23-396N6.2, AW546694, Bmi-1, Pcgf4)
Fusion protein or tag:
ires-eGFP
Terminal:
C terminal on insert
Vector backbone:
FUGW
(Search Vector Database)
Backbone manufacturer:
D. Baltimore
Vector type:
Lentiviral
Backbone size w/o insert (bp):
9941
Cloning site 5':
BamH1
Site destroyed during cloning:
Yes
Cloning site 3':
Not1 (partial digest)
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stable2 bacteria
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Sally Temple
Terms and Licenses:
MTA

Comments:

Bmi-1 cDNA cloned into pIRES-eGFP first. The Bmi1-ires-eGFP was then cut out and cloned into FUGW.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development. Fasano et al (Cell Stem Cell. 2007 Jun 7. 1(1):87-99. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21577" in your Materials and Methods section.