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pRetroX GFP T2A Cre
(Plasmid #63704)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63704 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRetrox
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7305
  • Total vector size (bp) 8424
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T2A Cre
  • Alt name
    Cre recombinase
  • Insert Size (bp)
    1124
  • Promoter tight TRE promoter
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX GFP T2A Cre was a gift from Floris Foijer (Addgene plasmid # 63704 ; http://n2t.net/addgene:63704 ; RRID:Addgene_63704)
  • For your References section:

    Chromosome instability induced by Mps1 and p53 mutation generates aggressive lymphomas exhibiting aneuploidy-induced stress. Foijer F, Xie SZ, Simon JE, Bakker PL, Conte N, Davis SH, Kregel E, Jonkers J, Bradley A, Sorger PK. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13427-32. doi: 10.1073/pnas.1400892111. Epub 2014 Sep 2. 10.1073/pnas.1400892111 PubMed 25197064