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(Plasmid #82477)


Item Catalog # Description Quantity Price (USD)
Plasmid 82477 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    SMX DEST vector Addgene 62399
  • Backbone size w/o insert (bp) 1950
  • Total vector size (bp) 10036
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    H2B-mCherry and H2B-Citrine. HygroR
  • Species
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer TTACCGCCTTTGAGTGAG
  • 3′ sequencing primer TTGTCTCATGAGCGGATAC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    miReporter-PGK was a gift from Pierre Neveu (Addgene plasmid # 82477 ; ; RRID:Addgene_82477)
  • For your References section:

    Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells. Sladitschek HL, Neveu PA. PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016. PONE-D-16-09196 [pii] PubMed 27152616