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DNMT3ACD-CRY2-EGFP
(Plasmid #82556)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82556 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV_EF1a_WPRE_hGHpA
  • Backbone manufacturer
    Zhang lab
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    DNMT3A
  • Species
    H. sapiens (human)
  • Entrez Gene
    DNMT3A (a.k.a. DNMT3A2, M.HsaIIIA, TBRS)
  • Promoter EF-1alpha

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Acc651 (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CRY2
  • Species
    A. thaliana (mustard weed)
  • Mutation
    CRY2PHR (N-terminal photolyase homology region domain of CRY2 only)
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Tags / Fusion Proteins
    • NLS(alpha-imp) (N terminal on backbone)
    • NLS-VP64 (C terminal on backbone)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    EGFP
  • Species
    jellyfish
  • Tag / Fusion Protein
    • 2A (N terminal on backbone)

Cloning Information for Gene/Insert 3

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DNMT3ACD-CRY2-EGFP was a gift from Joseph Irudayaraj (Addgene plasmid # 82556)