Purposehuman wild type DNA-PKcs
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83317||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4665
- Total vector size (bp) 17065
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionssee attached propagation instructions. This plasmid is prone to recombination. Transformed E. coli is extremely slow growing; please pick smallest colonies for a test prep, then check for appropriate EcoR1 restriction pattern.
Copy numberHigh Copy
Gene/Insert namehuman DNA-PKcs
SpeciesH. sapiens (human)
Insert Size (bp)12387
Entrez GenePRKDC (a.k.a. DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350)
- Promoter see attached information
- Cloning method Restriction Enzyme
- 5′ cloning site notI (unknown if destroyed)
- 3′ cloning site notI (unknown if destroyed)
- 5′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hu DNA-PKcs was a gift from Katheryn Meek (Addgene plasmid # 83317)
For your References section:Autophosphorylation of DNA-dependent protein kinase regulates DNA end processing and may also alter double-strand break repair pathway choice. Cui X, Yu Y, Gupta S, Cho YM, Lees-Miller SP, Meek K. Mol Cell Biol. 2005 Dec;25(24):10842-52. 10.1128/MCB.25.24.10842-10852.2005 PubMed 16314509