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hu DNA-PKcs
(Plasmid #83317)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83317 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcmv6
  • Backbone size w/o insert (bp) 4665
  • Total vector size (bp) 17065
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    see attached propagation instructions. This plasmid is prone to recombination. Transformed E. coli is extremely slow growing; please pick smallest colonies for a test prep, then check for appropriate EcoR1 restriction pattern.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human DNA-PKcs
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    12387
  • Mutation
    wild type
  • Entrez Gene
    PRKDC (a.k.a. DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350)
  • Promoter see attached information

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site notI (unknown if destroyed)
  • 3′ cloning site notI (unknown if destroyed)
  • 5′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hu DNA-PKcs was a gift from Katheryn Meek (Addgene plasmid # 83317)
  • For your References section:

    Autophosphorylation of DNA-dependent protein kinase regulates DNA end processing and may also alter double-strand break repair pathway choice. Cui X, Yu Y, Gupta S, Cho YM, Lees-Miller SP, Meek K. Mol Cell Biol. 2005 Dec;25(24):10842-52. 10.1128/MCB.25.24.10842-10852.2005 PubMed 16314509