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Tet-CA-Src-GFP
(Plasmid #83469)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83469 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    TRIPZ-non-silencing shRNA control
  • Backbone manufacturer
    GE-Healthcare
  • Backbone size w/o insert (bp) 12803
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin, Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Src
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2349
  • Mutation
    Y527F (constitutively active)
  • GenBank ID
    396442
  • Entrez Gene
    SRC (a.k.a. PP60C-SCR, SDR, c-src)
  • Promoter doxycycline-inducible promoter
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-CA-Src-GFP was a gift from Barry Gumbiner (Addgene plasmid # 83469 ; http://n2t.net/addgene:83469 ; RRID:Addgene_83469)
  • For your References section:

    Adhesion to fibronectin regulates Hippo signaling via the FAK-Src-PI3K pathway. Kim NG, Gumbiner BM. J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. Epub 2015 Jul 27. 10.1083/jcb.201501025 PubMed 26216901