pCMV-SHP2 CS
(Plasmid #8382)

• Depositing Lab: Ben Neel
• Publication: SHP2 plasmids (unpublished)

Backbone

• Vector backbone: pRC/CMV
• Backbone size w/o insert (bp): 5542
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SHP2
• Alt name: SH-PTP2
• Alt name: PTPN11
• Alt name: PTP2C
• Species: H. sapiens (human)
• Insert Size (bp): 2276
• Mutation: C459S, mutation of essential catalytic cysteine
• Entrez Gene: PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry